Fluorescent compounds are used in fluorescence microscopy to identify individual molecules or structures in a sample. Excitation light of a particular wavelength is absorbed by fluorescent molecules, which then emit emission light of a longer wavelength. The microscope then gathers the light that was emitted and uses it to make an image of the sample. When using a fluorescence microscope, chemicals that emit fluorescence are combined with the light microscope's magnification abilities. A high-intensity light source is used in fluorescence microscopy to excite a fluorophore, a fluorescent molecule, in the sample being examined. The following steps are involved in fluorescence microscopy:

1. Light with the desired excitation wavelength is produced by the light source.

2. The filter chooses the excitation wavelength and rejects all other light wavelengths.

3. Light strikes the specimen, causing the fluorescent molecules to light up.

4. The fluorescent molecules (emission wavelength) produce light with a larger wavelength.

5. The detector gathers the emitted light.

6. The detector magnifies the light that is being emitted and shows it as an image.

Fluorescent molecules are used in fluorescence microscopy, a type of microscopy that shows and measures the amount and distribution of particular molecules in a sample. Chongqing Scope Instrument Co., Ltd. has professional machines, technology and service. If you want more information, please click here.